48-well ecm array cell adhesion assay Search Results


matrigel  (Becton Dickinson)
90
Becton Dickinson matrigel
Matrigel, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/48-well+ecm+array+cell+adhesion+assay/matrigel/pmc07642114-98-8-11
Average 90 stars, based on 1 article reviews
matrigel - by Bioz Stars, 2026-09
90/100 stars
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lumos optical stimulator  (Axion BioSystems)
90
Axion BioSystems lumos optical stimulator
Lumos Optical Stimulator, supplied by Axion BioSystems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/48-well+ecm+array+cell+adhesion+assay/lumos+optical+stimulator/pmc07642238-515-22-17
Average 90 stars, based on 1 article reviews
lumos optical stimulator - by Bioz Stars, 2026-09
90/100 stars
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48-well meas  (Axion BioSystems)
90
Axion BioSystems 48-well meas
48 Well Meas, supplied by Axion BioSystems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/48-well+ecm+array+cell+adhesion+assay/cytoview+mea+24+well+plates/pm37861869-71-28-31
Average 90 stars, based on 1 article reviews
48-well meas - by Bioz Stars, 2026-09
90/100 stars
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48-well mea plates  (Axion BioSystems)
90
Axion BioSystems 48-well mea plates
( A ) ICC experimental timeline: neurons were plated on day in vitro (DIV) 0, virally transduced on DIV 2, and immunostained at DIV 15, DIV 22, or DIV 27. ( B ) Representative images of primary hippocampal cultures: untransduced (left), AAV-mKate2 (center), or AAV-BIN1-mKate2 (right), showing Tau and BIN1 immunostaining at DIV 15 (top), DIV 22 (middle), or DIV 27 (bottom). Scale bar = 20 µm. ( C ) AAV-BIN1-mKate2 increased BIN1 levels ~ 8–9-fold in BIN1 group compared to BIN1 levels in untransduced or mKate2 groups ( n = 2–6 fields of view per coverslip, 60x magnification, two-way ANOVA, BIN-DIV interaction p=0.1123, main effect of AAV-BIN1-mKate2 ****p<0.0001, main effect of DIV p=0.6373, Tukey’s multiple comparisons test: DIV 15:UnTD vs. DIV 15:AAV-BIN1-mKate2, ****p<0.0001, DIV 22:UnTD vs. DIV 22:AAV-BIN1-mKate2, ****p<0.0001, DIV 27:UnTD vs. DIV 27:AAV-BIN1-mKate2, ****p<0.0001. ( D ) Representative images of primary hippocampal cultures at DIV 12: untransduced (left), AAV-mKate2 (center), or AAV-BIN1-mKate2 (right), showing NeuN immunostaining (top), mKate2 fluorescence (middle), or merge of both (bottom). Scale bar = 25 µm. ( E ) The total number of neurons per well did not change between untransduced, mKate2, or BIN1 groups ( n = 6–7 coverslips, 10 × 10 fields of view per coverslip, 20x magnification, from two different primary neuron harvests, one-way ANOVA, p=0.5157). ( F ) <t>MEA</t> experimental timeline: neurons were plated on day in vitro (DIV) 0, virally transduced on DIV 2, and recorded on DIV 12. ( G ) Primary neuronal hippocampal cultures grown on an MEA plate. Scale bar = 50 µm. ( H ) Representative local field potential (LFP) traces. ( I ) Representative raster plots of firing activity from five different neurons per group. ( J ) BIN1 increased action potential frequency ( n = 27–36 neurons per group from three different primary neuron harvests, normalized to the controls from each harvest, median frequency in controls = 388 mHz; unpaired Mann-Whitney U test; p=0.0233). ( K ) BIN1 increased burst firing frequency ( n = 27–36 neurons per group from three different primary neuron harvests, normalized to the controls from each harvest, median frequency in controls = 11.7 mHz; unpaired Mann-Whitney U test; p=0.0020). ( L ) The total number of active neurons per well did not differ between mKate2 and BIN1 expressing groups ( n = 9 <t>MEA</t> <t>plates</t> for each group from three different primary neuron harvests, unpaired Student’s t test; p=0.346). All data are expressed as mean ± SEM, *p<0.05, **p<0.01, and ****p<0.0001. All data are expressed as mean ± SEM.
48 Well Mea Plates, supplied by Axion BioSystems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/48-well+ecm+array+cell+adhesion+assay/48+well+mea+plates/pmc07392604-174-15-17
Average 90 stars, based on 1 article reviews
48-well mea plates - by Bioz Stars, 2026-09
90/100 stars
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mea plate lumos 48  (Axion BioSystems)
90
Axion BioSystems mea plate lumos 48
( A ) ICC experimental timeline: neurons were plated on day in vitro (DIV) 0, virally transduced on DIV 2, and immunostained at DIV 15, DIV 22, or DIV 27. ( B ) Representative images of primary hippocampal cultures: untransduced (left), AAV-mKate2 (center), or AAV-BIN1-mKate2 (right), showing Tau and BIN1 immunostaining at DIV 15 (top), DIV 22 (middle), or DIV 27 (bottom). Scale bar = 20 µm. ( C ) AAV-BIN1-mKate2 increased BIN1 levels ~ 8–9-fold in BIN1 group compared to BIN1 levels in untransduced or mKate2 groups ( n = 2–6 fields of view per coverslip, 60x magnification, two-way ANOVA, BIN-DIV interaction p=0.1123, main effect of AAV-BIN1-mKate2 ****p<0.0001, main effect of DIV p=0.6373, Tukey’s multiple comparisons test: DIV 15:UnTD vs. DIV 15:AAV-BIN1-mKate2, ****p<0.0001, DIV 22:UnTD vs. DIV 22:AAV-BIN1-mKate2, ****p<0.0001, DIV 27:UnTD vs. DIV 27:AAV-BIN1-mKate2, ****p<0.0001. ( D ) Representative images of primary hippocampal cultures at DIV 12: untransduced (left), AAV-mKate2 (center), or AAV-BIN1-mKate2 (right), showing NeuN immunostaining (top), mKate2 fluorescence (middle), or merge of both (bottom). Scale bar = 25 µm. ( E ) The total number of neurons per well did not change between untransduced, mKate2, or BIN1 groups ( n = 6–7 coverslips, 10 × 10 fields of view per coverslip, 20x magnification, from two different primary neuron harvests, one-way ANOVA, p=0.5157). ( F ) <t>MEA</t> experimental timeline: neurons were plated on day in vitro (DIV) 0, virally transduced on DIV 2, and recorded on DIV 12. ( G ) Primary neuronal hippocampal cultures grown on an MEA plate. Scale bar = 50 µm. ( H ) Representative local field potential (LFP) traces. ( I ) Representative raster plots of firing activity from five different neurons per group. ( J ) BIN1 increased action potential frequency ( n = 27–36 neurons per group from three different primary neuron harvests, normalized to the controls from each harvest, median frequency in controls = 388 mHz; unpaired Mann-Whitney U test; p=0.0233). ( K ) BIN1 increased burst firing frequency ( n = 27–36 neurons per group from three different primary neuron harvests, normalized to the controls from each harvest, median frequency in controls = 11.7 mHz; unpaired Mann-Whitney U test; p=0.0020). ( L ) The total number of active neurons per well did not differ between mKate2 and BIN1 expressing groups ( n = 9 <t>MEA</t> <t>plates</t> for each group from three different primary neuron harvests, unpaired Student’s t test; p=0.346). All data are expressed as mean ± SEM, *p<0.05, **p<0.01, and ****p<0.0001. All data are expressed as mean ± SEM.
Mea Plate Lumos 48, supplied by Axion BioSystems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/48-well+ecm+array+cell+adhesion+assay/mea+plate+lumos+48/10__7554_slash_elife__50712-409-15-19
Average 90 stars, based on 1 article reviews
mea plate lumos 48 - by Bioz Stars, 2026-09
90/100 stars
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48-well slot blot manifold  (Cleaver Scientific)
90
Cleaver Scientific 48-well slot blot manifold
( A ) ICC experimental timeline: neurons were plated on day in vitro (DIV) 0, virally transduced on DIV 2, and immunostained at DIV 15, DIV 22, or DIV 27. ( B ) Representative images of primary hippocampal cultures: untransduced (left), AAV-mKate2 (center), or AAV-BIN1-mKate2 (right), showing Tau and BIN1 immunostaining at DIV 15 (top), DIV 22 (middle), or DIV 27 (bottom). Scale bar = 20 µm. ( C ) AAV-BIN1-mKate2 increased BIN1 levels ~ 8–9-fold in BIN1 group compared to BIN1 levels in untransduced or mKate2 groups ( n = 2–6 fields of view per coverslip, 60x magnification, two-way ANOVA, BIN-DIV interaction p=0.1123, main effect of AAV-BIN1-mKate2 ****p<0.0001, main effect of DIV p=0.6373, Tukey’s multiple comparisons test: DIV 15:UnTD vs. DIV 15:AAV-BIN1-mKate2, ****p<0.0001, DIV 22:UnTD vs. DIV 22:AAV-BIN1-mKate2, ****p<0.0001, DIV 27:UnTD vs. DIV 27:AAV-BIN1-mKate2, ****p<0.0001. ( D ) Representative images of primary hippocampal cultures at DIV 12: untransduced (left), AAV-mKate2 (center), or AAV-BIN1-mKate2 (right), showing NeuN immunostaining (top), mKate2 fluorescence (middle), or merge of both (bottom). Scale bar = 25 µm. ( E ) The total number of neurons per well did not change between untransduced, mKate2, or BIN1 groups ( n = 6–7 coverslips, 10 × 10 fields of view per coverslip, 20x magnification, from two different primary neuron harvests, one-way ANOVA, p=0.5157). ( F ) <t>MEA</t> experimental timeline: neurons were plated on day in vitro (DIV) 0, virally transduced on DIV 2, and recorded on DIV 12. ( G ) Primary neuronal hippocampal cultures grown on an MEA plate. Scale bar = 50 µm. ( H ) Representative local field potential (LFP) traces. ( I ) Representative raster plots of firing activity from five different neurons per group. ( J ) BIN1 increased action potential frequency ( n = 27–36 neurons per group from three different primary neuron harvests, normalized to the controls from each harvest, median frequency in controls = 388 mHz; unpaired Mann-Whitney U test; p=0.0233). ( K ) BIN1 increased burst firing frequency ( n = 27–36 neurons per group from three different primary neuron harvests, normalized to the controls from each harvest, median frequency in controls = 11.7 mHz; unpaired Mann-Whitney U test; p=0.0020). ( L ) The total number of active neurons per well did not differ between mKate2 and BIN1 expressing groups ( n = 9 <t>MEA</t> <t>plates</t> for each group from three different primary neuron harvests, unpaired Student’s t test; p=0.346). All data are expressed as mean ± SEM, *p<0.05, **p<0.01, and ****p<0.0001. All data are expressed as mean ± SEM.
48 Well Slot Blot Manifold, supplied by Cleaver Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/48-well+ecm+array+cell+adhesion+assay/48+well+slot+blot+manifold/pmc06940912-147-11-15
Average 90 stars, based on 1 article reviews
48-well slot blot manifold - by Bioz Stars, 2026-09
90/100 stars
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multichem 48-well synthesis apparatus  (SciGene Inc)
90
SciGene Inc multichem 48-well synthesis apparatus
( A ) ICC experimental timeline: neurons were plated on day in vitro (DIV) 0, virally transduced on DIV 2, and immunostained at DIV 15, DIV 22, or DIV 27. ( B ) Representative images of primary hippocampal cultures: untransduced (left), AAV-mKate2 (center), or AAV-BIN1-mKate2 (right), showing Tau and BIN1 immunostaining at DIV 15 (top), DIV 22 (middle), or DIV 27 (bottom). Scale bar = 20 µm. ( C ) AAV-BIN1-mKate2 increased BIN1 levels ~ 8–9-fold in BIN1 group compared to BIN1 levels in untransduced or mKate2 groups ( n = 2–6 fields of view per coverslip, 60x magnification, two-way ANOVA, BIN-DIV interaction p=0.1123, main effect of AAV-BIN1-mKate2 ****p<0.0001, main effect of DIV p=0.6373, Tukey’s multiple comparisons test: DIV 15:UnTD vs. DIV 15:AAV-BIN1-mKate2, ****p<0.0001, DIV 22:UnTD vs. DIV 22:AAV-BIN1-mKate2, ****p<0.0001, DIV 27:UnTD vs. DIV 27:AAV-BIN1-mKate2, ****p<0.0001. ( D ) Representative images of primary hippocampal cultures at DIV 12: untransduced (left), AAV-mKate2 (center), or AAV-BIN1-mKate2 (right), showing NeuN immunostaining (top), mKate2 fluorescence (middle), or merge of both (bottom). Scale bar = 25 µm. ( E ) The total number of neurons per well did not change between untransduced, mKate2, or BIN1 groups ( n = 6–7 coverslips, 10 × 10 fields of view per coverslip, 20x magnification, from two different primary neuron harvests, one-way ANOVA, p=0.5157). ( F ) <t>MEA</t> experimental timeline: neurons were plated on day in vitro (DIV) 0, virally transduced on DIV 2, and recorded on DIV 12. ( G ) Primary neuronal hippocampal cultures grown on an MEA plate. Scale bar = 50 µm. ( H ) Representative local field potential (LFP) traces. ( I ) Representative raster plots of firing activity from five different neurons per group. ( J ) BIN1 increased action potential frequency ( n = 27–36 neurons per group from three different primary neuron harvests, normalized to the controls from each harvest, median frequency in controls = 388 mHz; unpaired Mann-Whitney U test; p=0.0233). ( K ) BIN1 increased burst firing frequency ( n = 27–36 neurons per group from three different primary neuron harvests, normalized to the controls from each harvest, median frequency in controls = 11.7 mHz; unpaired Mann-Whitney U test; p=0.0020). ( L ) The total number of active neurons per well did not differ between mKate2 and BIN1 expressing groups ( n = 9 <t>MEA</t> <t>plates</t> for each group from three different primary neuron harvests, unpaired Student’s t test; p=0.346). All data are expressed as mean ± SEM, *p<0.05, **p<0.01, and ****p<0.0001. All data are expressed as mean ± SEM.
Multichem 48 Well Synthesis Apparatus, supplied by SciGene Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/48-well+ecm+array+cell+adhesion+assay/multichem+48+well+synthesis+apparatus/10__1038_slash_nprot__2017__091-307-67-72
Average 90 stars, based on 1 article reviews
multichem 48-well synthesis apparatus - by Bioz Stars, 2026-09
90/100 stars
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non-tissue culture-treated, polystyrene 48-well cluster plates  (Corning Life Sciences)
90
Corning Life Sciences non-tissue culture-treated, polystyrene 48-well cluster plates
( A ) ICC experimental timeline: neurons were plated on day in vitro (DIV) 0, virally transduced on DIV 2, and immunostained at DIV 15, DIV 22, or DIV 27. ( B ) Representative images of primary hippocampal cultures: untransduced (left), AAV-mKate2 (center), or AAV-BIN1-mKate2 (right), showing Tau and BIN1 immunostaining at DIV 15 (top), DIV 22 (middle), or DIV 27 (bottom). Scale bar = 20 µm. ( C ) AAV-BIN1-mKate2 increased BIN1 levels ~ 8–9-fold in BIN1 group compared to BIN1 levels in untransduced or mKate2 groups ( n = 2–6 fields of view per coverslip, 60x magnification, two-way ANOVA, BIN-DIV interaction p=0.1123, main effect of AAV-BIN1-mKate2 ****p<0.0001, main effect of DIV p=0.6373, Tukey’s multiple comparisons test: DIV 15:UnTD vs. DIV 15:AAV-BIN1-mKate2, ****p<0.0001, DIV 22:UnTD vs. DIV 22:AAV-BIN1-mKate2, ****p<0.0001, DIV 27:UnTD vs. DIV 27:AAV-BIN1-mKate2, ****p<0.0001. ( D ) Representative images of primary hippocampal cultures at DIV 12: untransduced (left), AAV-mKate2 (center), or AAV-BIN1-mKate2 (right), showing NeuN immunostaining (top), mKate2 fluorescence (middle), or merge of both (bottom). Scale bar = 25 µm. ( E ) The total number of neurons per well did not change between untransduced, mKate2, or BIN1 groups ( n = 6–7 coverslips, 10 × 10 fields of view per coverslip, 20x magnification, from two different primary neuron harvests, one-way ANOVA, p=0.5157). ( F ) <t>MEA</t> experimental timeline: neurons were plated on day in vitro (DIV) 0, virally transduced on DIV 2, and recorded on DIV 12. ( G ) Primary neuronal hippocampal cultures grown on an MEA plate. Scale bar = 50 µm. ( H ) Representative local field potential (LFP) traces. ( I ) Representative raster plots of firing activity from five different neurons per group. ( J ) BIN1 increased action potential frequency ( n = 27–36 neurons per group from three different primary neuron harvests, normalized to the controls from each harvest, median frequency in controls = 388 mHz; unpaired Mann-Whitney U test; p=0.0233). ( K ) BIN1 increased burst firing frequency ( n = 27–36 neurons per group from three different primary neuron harvests, normalized to the controls from each harvest, median frequency in controls = 11.7 mHz; unpaired Mann-Whitney U test; p=0.0020). ( L ) The total number of active neurons per well did not differ between mKate2 and BIN1 expressing groups ( n = 9 <t>MEA</t> <t>plates</t> for each group from three different primary neuron harvests, unpaired Student’s t test; p=0.346). All data are expressed as mean ± SEM, *p<0.05, **p<0.01, and ****p<0.0001. All data are expressed as mean ± SEM.
Non Tissue Culture Treated, Polystyrene 48 Well Cluster Plates, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/48-well+ecm+array+cell+adhesion+assay/non+tissue+culture+treated++polystyrene+48+well+cluster+plates/pm30361699-466-3-7
Average 90 stars, based on 1 article reviews
non-tissue culture-treated, polystyrene 48-well cluster plates - by Bioz Stars, 2026-09
90/100 stars
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48 well plates  (Thermo Fisher)
99
Thermo Fisher 48 well plates
( A ) ICC experimental timeline: neurons were plated on day in vitro (DIV) 0, virally transduced on DIV 2, and immunostained at DIV 15, DIV 22, or DIV 27. ( B ) Representative images of primary hippocampal cultures: untransduced (left), AAV-mKate2 (center), or AAV-BIN1-mKate2 (right), showing Tau and BIN1 immunostaining at DIV 15 (top), DIV 22 (middle), or DIV 27 (bottom). Scale bar = 20 µm. ( C ) AAV-BIN1-mKate2 increased BIN1 levels ~ 8–9-fold in BIN1 group compared to BIN1 levels in untransduced or mKate2 groups ( n = 2–6 fields of view per coverslip, 60x magnification, two-way ANOVA, BIN-DIV interaction p=0.1123, main effect of AAV-BIN1-mKate2 ****p<0.0001, main effect of DIV p=0.6373, Tukey’s multiple comparisons test: DIV 15:UnTD vs. DIV 15:AAV-BIN1-mKate2, ****p<0.0001, DIV 22:UnTD vs. DIV 22:AAV-BIN1-mKate2, ****p<0.0001, DIV 27:UnTD vs. DIV 27:AAV-BIN1-mKate2, ****p<0.0001. ( D ) Representative images of primary hippocampal cultures at DIV 12: untransduced (left), AAV-mKate2 (center), or AAV-BIN1-mKate2 (right), showing NeuN immunostaining (top), mKate2 fluorescence (middle), or merge of both (bottom). Scale bar = 25 µm. ( E ) The total number of neurons per well did not change between untransduced, mKate2, or BIN1 groups ( n = 6–7 coverslips, 10 × 10 fields of view per coverslip, 20x magnification, from two different primary neuron harvests, one-way ANOVA, p=0.5157). ( F ) <t>MEA</t> experimental timeline: neurons were plated on day in vitro (DIV) 0, virally transduced on DIV 2, and recorded on DIV 12. ( G ) Primary neuronal hippocampal cultures grown on an MEA plate. Scale bar = 50 µm. ( H ) Representative local field potential (LFP) traces. ( I ) Representative raster plots of firing activity from five different neurons per group. ( J ) BIN1 increased action potential frequency ( n = 27–36 neurons per group from three different primary neuron harvests, normalized to the controls from each harvest, median frequency in controls = 388 mHz; unpaired Mann-Whitney U test; p=0.0233). ( K ) BIN1 increased burst firing frequency ( n = 27–36 neurons per group from three different primary neuron harvests, normalized to the controls from each harvest, median frequency in controls = 11.7 mHz; unpaired Mann-Whitney U test; p=0.0020). ( L ) The total number of active neurons per well did not differ between mKate2 and BIN1 expressing groups ( n = 9 <t>MEA</t> <t>plates</t> for each group from three different primary neuron harvests, unpaired Student’s t test; p=0.346). All data are expressed as mean ± SEM, *p<0.05, **p<0.01, and ****p<0.0001. All data are expressed as mean ± SEM.
48 Well Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/48-well+ecm+array+cell+adhesion+assay/Collagen/pmc05898985-483-7-19
Average 99 stars, based on 1 article reviews
48 well plates - by Bioz Stars, 2026-09
99/100 stars
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vitek ms disposable target slide  (bioMerieux gmbh)
90
bioMerieux gmbh vitek ms disposable target slide
( A ) ICC experimental timeline: neurons were plated on day in vitro (DIV) 0, virally transduced on DIV 2, and immunostained at DIV 15, DIV 22, or DIV 27. ( B ) Representative images of primary hippocampal cultures: untransduced (left), AAV-mKate2 (center), or AAV-BIN1-mKate2 (right), showing Tau and BIN1 immunostaining at DIV 15 (top), DIV 22 (middle), or DIV 27 (bottom). Scale bar = 20 µm. ( C ) AAV-BIN1-mKate2 increased BIN1 levels ~ 8–9-fold in BIN1 group compared to BIN1 levels in untransduced or mKate2 groups ( n = 2–6 fields of view per coverslip, 60x magnification, two-way ANOVA, BIN-DIV interaction p=0.1123, main effect of AAV-BIN1-mKate2 ****p<0.0001, main effect of DIV p=0.6373, Tukey’s multiple comparisons test: DIV 15:UnTD vs. DIV 15:AAV-BIN1-mKate2, ****p<0.0001, DIV 22:UnTD vs. DIV 22:AAV-BIN1-mKate2, ****p<0.0001, DIV 27:UnTD vs. DIV 27:AAV-BIN1-mKate2, ****p<0.0001. ( D ) Representative images of primary hippocampal cultures at DIV 12: untransduced (left), AAV-mKate2 (center), or AAV-BIN1-mKate2 (right), showing NeuN immunostaining (top), mKate2 fluorescence (middle), or merge of both (bottom). Scale bar = 25 µm. ( E ) The total number of neurons per well did not change between untransduced, mKate2, or BIN1 groups ( n = 6–7 coverslips, 10 × 10 fields of view per coverslip, 20x magnification, from two different primary neuron harvests, one-way ANOVA, p=0.5157). ( F ) <t>MEA</t> experimental timeline: neurons were plated on day in vitro (DIV) 0, virally transduced on DIV 2, and recorded on DIV 12. ( G ) Primary neuronal hippocampal cultures grown on an MEA plate. Scale bar = 50 µm. ( H ) Representative local field potential (LFP) traces. ( I ) Representative raster plots of firing activity from five different neurons per group. ( J ) BIN1 increased action potential frequency ( n = 27–36 neurons per group from three different primary neuron harvests, normalized to the controls from each harvest, median frequency in controls = 388 mHz; unpaired Mann-Whitney U test; p=0.0233). ( K ) BIN1 increased burst firing frequency ( n = 27–36 neurons per group from three different primary neuron harvests, normalized to the controls from each harvest, median frequency in controls = 11.7 mHz; unpaired Mann-Whitney U test; p=0.0020). ( L ) The total number of active neurons per well did not differ between mKate2 and BIN1 expressing groups ( n = 9 <t>MEA</t> <t>plates</t> for each group from three different primary neuron harvests, unpaired Student’s t test; p=0.346). All data are expressed as mean ± SEM, *p<0.05, **p<0.01, and ****p<0.0001. All data are expressed as mean ± SEM.
Vitek Ms Disposable Target Slide, supplied by bioMerieux gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/48-well+ecm+array+cell+adhesion+assay/vitek+ms+target+plate/pmc07591085-61-16-32
Average 90 stars, based on 1 article reviews
vitek ms disposable target slide - by Bioz Stars, 2026-09
90/100 stars
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trans-well inserts (48-well, pore)  (Corning Life Sciences)
90
Corning Life Sciences trans-well inserts (48-well, pore)
( A ) ICC experimental timeline: neurons were plated on day in vitro (DIV) 0, virally transduced on DIV 2, and immunostained at DIV 15, DIV 22, or DIV 27. ( B ) Representative images of primary hippocampal cultures: untransduced (left), AAV-mKate2 (center), or AAV-BIN1-mKate2 (right), showing Tau and BIN1 immunostaining at DIV 15 (top), DIV 22 (middle), or DIV 27 (bottom). Scale bar = 20 µm. ( C ) AAV-BIN1-mKate2 increased BIN1 levels ~ 8–9-fold in BIN1 group compared to BIN1 levels in untransduced or mKate2 groups ( n = 2–6 fields of view per coverslip, 60x magnification, two-way ANOVA, BIN-DIV interaction p=0.1123, main effect of AAV-BIN1-mKate2 ****p<0.0001, main effect of DIV p=0.6373, Tukey’s multiple comparisons test: DIV 15:UnTD vs. DIV 15:AAV-BIN1-mKate2, ****p<0.0001, DIV 22:UnTD vs. DIV 22:AAV-BIN1-mKate2, ****p<0.0001, DIV 27:UnTD vs. DIV 27:AAV-BIN1-mKate2, ****p<0.0001. ( D ) Representative images of primary hippocampal cultures at DIV 12: untransduced (left), AAV-mKate2 (center), or AAV-BIN1-mKate2 (right), showing NeuN immunostaining (top), mKate2 fluorescence (middle), or merge of both (bottom). Scale bar = 25 µm. ( E ) The total number of neurons per well did not change between untransduced, mKate2, or BIN1 groups ( n = 6–7 coverslips, 10 × 10 fields of view per coverslip, 20x magnification, from two different primary neuron harvests, one-way ANOVA, p=0.5157). ( F ) <t>MEA</t> experimental timeline: neurons were plated on day in vitro (DIV) 0, virally transduced on DIV 2, and recorded on DIV 12. ( G ) Primary neuronal hippocampal cultures grown on an MEA plate. Scale bar = 50 µm. ( H ) Representative local field potential (LFP) traces. ( I ) Representative raster plots of firing activity from five different neurons per group. ( J ) BIN1 increased action potential frequency ( n = 27–36 neurons per group from three different primary neuron harvests, normalized to the controls from each harvest, median frequency in controls = 388 mHz; unpaired Mann-Whitney U test; p=0.0233). ( K ) BIN1 increased burst firing frequency ( n = 27–36 neurons per group from three different primary neuron harvests, normalized to the controls from each harvest, median frequency in controls = 11.7 mHz; unpaired Mann-Whitney U test; p=0.0020). ( L ) The total number of active neurons per well did not differ between mKate2 and BIN1 expressing groups ( n = 9 <t>MEA</t> <t>plates</t> for each group from three different primary neuron harvests, unpaired Student’s t test; p=0.346). All data are expressed as mean ± SEM, *p<0.05, **p<0.01, and ****p<0.0001. All data are expressed as mean ± SEM.
Trans Well Inserts (48 Well, Pore), supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/48-well+ecm+array+cell+adhesion+assay/96+well+plates/us09687483-224-35-41
Average 90 stars, based on 1 article reviews
trans-well inserts (48-well, pore) - by Bioz Stars, 2026-09
90/100 stars
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falcon multiwell 48-well tissue culture plates  (Becton Dickinson)
90
Becton Dickinson falcon multiwell 48-well tissue culture plates
( A ) ICC experimental timeline: neurons were plated on day in vitro (DIV) 0, virally transduced on DIV 2, and immunostained at DIV 15, DIV 22, or DIV 27. ( B ) Representative images of primary hippocampal cultures: untransduced (left), AAV-mKate2 (center), or AAV-BIN1-mKate2 (right), showing Tau and BIN1 immunostaining at DIV 15 (top), DIV 22 (middle), or DIV 27 (bottom). Scale bar = 20 µm. ( C ) AAV-BIN1-mKate2 increased BIN1 levels ~ 8–9-fold in BIN1 group compared to BIN1 levels in untransduced or mKate2 groups ( n = 2–6 fields of view per coverslip, 60x magnification, two-way ANOVA, BIN-DIV interaction p=0.1123, main effect of AAV-BIN1-mKate2 ****p<0.0001, main effect of DIV p=0.6373, Tukey’s multiple comparisons test: DIV 15:UnTD vs. DIV 15:AAV-BIN1-mKate2, ****p<0.0001, DIV 22:UnTD vs. DIV 22:AAV-BIN1-mKate2, ****p<0.0001, DIV 27:UnTD vs. DIV 27:AAV-BIN1-mKate2, ****p<0.0001. ( D ) Representative images of primary hippocampal cultures at DIV 12: untransduced (left), AAV-mKate2 (center), or AAV-BIN1-mKate2 (right), showing NeuN immunostaining (top), mKate2 fluorescence (middle), or merge of both (bottom). Scale bar = 25 µm. ( E ) The total number of neurons per well did not change between untransduced, mKate2, or BIN1 groups ( n = 6–7 coverslips, 10 × 10 fields of view per coverslip, 20x magnification, from two different primary neuron harvests, one-way ANOVA, p=0.5157). ( F ) <t>MEA</t> experimental timeline: neurons were plated on day in vitro (DIV) 0, virally transduced on DIV 2, and recorded on DIV 12. ( G ) Primary neuronal hippocampal cultures grown on an MEA plate. Scale bar = 50 µm. ( H ) Representative local field potential (LFP) traces. ( I ) Representative raster plots of firing activity from five different neurons per group. ( J ) BIN1 increased action potential frequency ( n = 27–36 neurons per group from three different primary neuron harvests, normalized to the controls from each harvest, median frequency in controls = 388 mHz; unpaired Mann-Whitney U test; p=0.0233). ( K ) BIN1 increased burst firing frequency ( n = 27–36 neurons per group from three different primary neuron harvests, normalized to the controls from each harvest, median frequency in controls = 11.7 mHz; unpaired Mann-Whitney U test; p=0.0020). ( L ) The total number of active neurons per well did not differ between mKate2 and BIN1 expressing groups ( n = 9 <t>MEA</t> <t>plates</t> for each group from three different primary neuron harvests, unpaired Student’s t test; p=0.346). All data are expressed as mean ± SEM, *p<0.05, **p<0.01, and ****p<0.0001. All data are expressed as mean ± SEM.
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( A ) ICC experimental timeline: neurons were plated on day in vitro (DIV) 0, virally transduced on DIV 2, and immunostained at DIV 15, DIV 22, or DIV 27. ( B ) Representative images of primary hippocampal cultures: untransduced (left), AAV-mKate2 (center), or AAV-BIN1-mKate2 (right), showing Tau and BIN1 immunostaining at DIV 15 (top), DIV 22 (middle), or DIV 27 (bottom). Scale bar = 20 µm. ( C ) AAV-BIN1-mKate2 increased BIN1 levels ~ 8–9-fold in BIN1 group compared to BIN1 levels in untransduced or mKate2 groups ( n = 2–6 fields of view per coverslip, 60x magnification, two-way ANOVA, BIN-DIV interaction p=0.1123, main effect of AAV-BIN1-mKate2 ****p<0.0001, main effect of DIV p=0.6373, Tukey’s multiple comparisons test: DIV 15:UnTD vs. DIV 15:AAV-BIN1-mKate2, ****p<0.0001, DIV 22:UnTD vs. DIV 22:AAV-BIN1-mKate2, ****p<0.0001, DIV 27:UnTD vs. DIV 27:AAV-BIN1-mKate2, ****p<0.0001. ( D ) Representative images of primary hippocampal cultures at DIV 12: untransduced (left), AAV-mKate2 (center), or AAV-BIN1-mKate2 (right), showing NeuN immunostaining (top), mKate2 fluorescence (middle), or merge of both (bottom). Scale bar = 25 µm. ( E ) The total number of neurons per well did not change between untransduced, mKate2, or BIN1 groups ( n = 6–7 coverslips, 10 × 10 fields of view per coverslip, 20x magnification, from two different primary neuron harvests, one-way ANOVA, p=0.5157). ( F ) MEA experimental timeline: neurons were plated on day in vitro (DIV) 0, virally transduced on DIV 2, and recorded on DIV 12. ( G ) Primary neuronal hippocampal cultures grown on an MEA plate. Scale bar = 50 µm. ( H ) Representative local field potential (LFP) traces. ( I ) Representative raster plots of firing activity from five different neurons per group. ( J ) BIN1 increased action potential frequency ( n = 27–36 neurons per group from three different primary neuron harvests, normalized to the controls from each harvest, median frequency in controls = 388 mHz; unpaired Mann-Whitney U test; p=0.0233). ( K ) BIN1 increased burst firing frequency ( n = 27–36 neurons per group from three different primary neuron harvests, normalized to the controls from each harvest, median frequency in controls = 11.7 mHz; unpaired Mann-Whitney U test; p=0.0020). ( L ) The total number of active neurons per well did not differ between mKate2 and BIN1 expressing groups ( n = 9 MEA plates for each group from three different primary neuron harvests, unpaired Student’s t test; p=0.346). All data are expressed as mean ± SEM, *p<0.05, **p<0.01, and ****p<0.0001. All data are expressed as mean ± SEM.

Journal: eLife

Article Title: Alzheimer’s disease risk gene BIN1 induces Tau-dependent network hyperexcitability

doi: 10.7554/eLife.57354

Figure Lengend Snippet: ( A ) ICC experimental timeline: neurons were plated on day in vitro (DIV) 0, virally transduced on DIV 2, and immunostained at DIV 15, DIV 22, or DIV 27. ( B ) Representative images of primary hippocampal cultures: untransduced (left), AAV-mKate2 (center), or AAV-BIN1-mKate2 (right), showing Tau and BIN1 immunostaining at DIV 15 (top), DIV 22 (middle), or DIV 27 (bottom). Scale bar = 20 µm. ( C ) AAV-BIN1-mKate2 increased BIN1 levels ~ 8–9-fold in BIN1 group compared to BIN1 levels in untransduced or mKate2 groups ( n = 2–6 fields of view per coverslip, 60x magnification, two-way ANOVA, BIN-DIV interaction p=0.1123, main effect of AAV-BIN1-mKate2 ****p<0.0001, main effect of DIV p=0.6373, Tukey’s multiple comparisons test: DIV 15:UnTD vs. DIV 15:AAV-BIN1-mKate2, ****p<0.0001, DIV 22:UnTD vs. DIV 22:AAV-BIN1-mKate2, ****p<0.0001, DIV 27:UnTD vs. DIV 27:AAV-BIN1-mKate2, ****p<0.0001. ( D ) Representative images of primary hippocampal cultures at DIV 12: untransduced (left), AAV-mKate2 (center), or AAV-BIN1-mKate2 (right), showing NeuN immunostaining (top), mKate2 fluorescence (middle), or merge of both (bottom). Scale bar = 25 µm. ( E ) The total number of neurons per well did not change between untransduced, mKate2, or BIN1 groups ( n = 6–7 coverslips, 10 × 10 fields of view per coverslip, 20x magnification, from two different primary neuron harvests, one-way ANOVA, p=0.5157). ( F ) MEA experimental timeline: neurons were plated on day in vitro (DIV) 0, virally transduced on DIV 2, and recorded on DIV 12. ( G ) Primary neuronal hippocampal cultures grown on an MEA plate. Scale bar = 50 µm. ( H ) Representative local field potential (LFP) traces. ( I ) Representative raster plots of firing activity from five different neurons per group. ( J ) BIN1 increased action potential frequency ( n = 27–36 neurons per group from three different primary neuron harvests, normalized to the controls from each harvest, median frequency in controls = 388 mHz; unpaired Mann-Whitney U test; p=0.0233). ( K ) BIN1 increased burst firing frequency ( n = 27–36 neurons per group from three different primary neuron harvests, normalized to the controls from each harvest, median frequency in controls = 11.7 mHz; unpaired Mann-Whitney U test; p=0.0020). ( L ) The total number of active neurons per well did not differ between mKate2 and BIN1 expressing groups ( n = 9 MEA plates for each group from three different primary neuron harvests, unpaired Student’s t test; p=0.346). All data are expressed as mean ± SEM, *p<0.05, **p<0.01, and ****p<0.0001. All data are expressed as mean ± SEM.

Article Snippet: For 48-well plate multielectrode array recordings, neurons were plated at 30,000 per well in 48-well MEA plates (Axion Biosystems, M768-tMEA-48B-5).

Techniques: In Vitro, Immunostaining, Fluorescence, Activity Assay, MANN-WHITNEY, Expressing